Propidium Iodide (PI) Staining Solution (10 µm)

• 1. Prepare a 1 mm stock solution of propidium iodide (PI) (Invitrogen P3566; 1.0 mg/mL) in H₂O. Cover in aluminum foil to minimize light exposure. Store at 2°C–8°C.

• 2. Immediately before use, dilute the stock solution with spring water to prepare a staining solution of 10 µm PI.

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