Recipe

PNK (1×) + EGTA Buffer

Reagent Quantity (for 1 L) Final concentration
Tris-HCl (1 m, pH 7.4) 50 mL   50 mm
EGTA (0.5 m) 40 mL   20 mm
Nonidet P-40 (NP-40)  5 mL 0.5% (v/v)
Make up to 1 L with water. Store at 4°C.
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  1. doi:10.1101/pdb.rec099408 Cold Spring Harb Protoc 2018: pdb.rec099408- © 2018 Cold Spring Harbor Laboratory Press
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