High-Stringency Immunoprecipitation Wash Buffer
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Tris-HCl (1 m, pH 7.5) | 15 mL | 15 mm |
| EDTA (0.5 m, pH 8.0) | 10 mL | 5 mm |
| EGTA (0.5 m) | 5 mL | 2.5 mm |
| Triton X-100 | 10 mL | 1% (v/v) |
| Na-deoxycholate (DOC) | 10 g | 1% (w/v) |
| SDS (20%) | 5 mL | 0.1% (v/v) |
| NaCl (5.0 m) | 24 mL | 120 mm |
| KCl (1.0 m) | 25 mL | 25 mm |
| Make up to 1 L with RNase-free water. Store at 4°C. | ||
