Mild Immunoprecipitation Wash Buffer
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| HEPES (1 m, pH 7.4 at room temperature) | 20 mL | 20 mm |
| NaCl (5 m) | 30 mL | 150 mm |
| MgCl2 (1 m) | 5 mL | 5 mm |
| Nonidet P-40 (NP-40) | 10 mL | 1% (v/v) |
| Make up to 1 L with RNase-free water. Store at 4°C. | ||
