Loading Buffer for Denaturing Polyacrylamide Gels (2×)
| Reagent | Quantity (for 1 mL) | Final concentration |
|---|---|---|
| Formamide, deionized | 950 µL | 95% (v/v) |
| EDTA (100 mm, pH 8) | 50 µL | 5% (v/v) |
| Add four parts formamide/EDTA mix to 1 part 0.5% solution of bromophenol blue and xylene cyanol (Sigma-Aldrich B-3269) mixed in water. Store at −20°C. | ||
