Denaturing Polyacrylamide Gel
| Reagent | Quantity (for 20 mL) | Final concentration |
|---|---|---|
| Urea | 8.4 g | 7 m |
| Acrylamide:bisacrylamide (19:1; 40% stock) | 5 mL | 10% (v/v) |
| TBE buffer (5×) | 4 mL | 1× |
| Ammonium persulfate (10% w/v) | 200 µL | 0.1% (w/v) |
| TEMED | 7.5 µL | 0.0375% (v/v) |
| Twenty milliliters of mix is made to pour a 1.5-mm-thick gel. Immediately before pouring the gel, add APS and TEMED. | ||
