Recipe

Tricaine (5×) in Holtfreter's Solution

Reagent Quantity for 500 mL of 5× solution Final concentration (5×)
NaCl  8.75 g        300 mm
KCl 0.125 g         3 mm
CaCl2  0.25 g       4.5 mm
NaHCO3 0.500 g      12.5 mm
Tricaine (ethyl 3-aminobenzonate methanesulfonate)  1.25 g 2.5 mg/mL (0.25%)
To prepare 500 mL, dissolve all reagents in 500 mL deionized H2O. Sterilization is not required. Store at 4°C.
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  1. doi:10.1101/pdb.rec104661 Cold Spring Harb Protoc 2018: pdb.rec104661- © 2018 Cold Spring Harbor Laboratory Press
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