Tricaine (5×) in Holtfreter's Solution
| Reagent | Quantity for 500 mL of 5× solution | Final concentration (5×) |
|---|---|---|
| NaCl | 8.75 g | 300 mm |
| KCl | 0.125 g | 3 mm |
| CaCl2 | 0.25 g | 4.5 mm |
| NaHCO3 | 0.500 g | 12.5 mm |
| Tricaine (ethyl 3-aminobenzonate methanesulfonate) | 1.25 g | 2.5 mg/mL (0.25%) |
| To prepare 500 mL, dissolve all reagents in 500 mL deionized H2O. Sterilization is not required. Store at 4°C. | ||
