Modified Barth's Solution (MBS) (1×)
| Reagent | Final concentration (1×) |
|---|---|
| CaCl2 | 0.41 mm |
| Ca(NO3)2 | 0.33 mm |
| HEPES | 10 mm |
| KCl | 1.0 mm |
| MgSO4 | 0.82 mm |
| NaCl | 88 mm |
| NaHCO3 | 2.4 mm |
| Adjust the pH to 7.4. Prepare as a 10× solution and then autoclave. Store at 4°C for up to 1 mo. Dilute to 0.1× and add antibiotics before use, and store for up to 3 d. | |
