Protocol

Preparation of Antisense Oligonucleotides to Inhibit miRNA Function

  1. Phillip D. Zamore

Abstract

This protocol is used to design antisense oligonucleotides (ASOs) for specific inhibition of miRNA function in cultured cells. The incorporation of 2′-O-methyl-modifications in ASOs enhances their potency and resistance to degradation, whereas 3′-terminal cholesterol-conjugation facilitates delivery of ASOs into cells.

Footnotes

  • From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.

| Table of Contents

This Article

  1. doi:10.1101/pdb.prot097527 Cold Spring Harb Protoc 2018: pdb.prot097527- © 2018 Cold Spring Harbor Laboratory Press
  1. » Abstract
  2. Full Text
  3. Full Text (PDF)

Article Category

  1. Protocol

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue