Oocyte Culture Medium (OCM)
| Reagent | Amount to add | Final concentration |
|---|---|---|
| Leibovitz's L-15 medium with l-glutamine | 0.35 L | 70% |
| Bovine serum albumin (fraction V) | 0.2 g | 0.4 mg/mL |
| Penicillin-streptomycin (100×/100×, P4333 Sigma-Aldrich) | 5 mL | 100 U/mL penicillin |
| 0.1 mg/mL streptomycin | ||
| Bring the volume to 0.5 L with deionized H2O, and adjust the pH to 7.8 with NaOH. Filter to sterilize. Store at 4°C–8°C for up to 1–2 mo. Check before use. | ||
