Figure 2.
Figure 2.

A schematic representation of a genetic cross using the PEM system. cyhS and cyhR are cycloheximide (CYH)-sensitive (rpl42+) and -resistant (rpl42-P56Q) alleles, respectively. YFG::NAT and Lib::G418 are query and library deletions, respectively. MTL, mating-type locus; ADS, antidiploid selection; MTS, mating-type selection; DMS, double-mutant selection.

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  1. Published in Advance July 21, 2017, doi: 10.1101/pdb.top079905 Cold Spring Harb Protoc 2018: pdb.top079905-
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