TABLE 1.
Antibodies
| No | Antibody | Detection | Dilution |
|---|---|---|---|
| 1 | Mouse anti-BrdU (BD Biosciences 347580) | IdU and BrdU | 1:20 |
| 2 | Rabbit anti-ssDNA (IBL 18731) | ssDNA | 1:50 |
| 3 | Rat anti-BrdU (Abcam ab 6326) | CldU and BrdU | 1:50 |
| 4 | Alexa fluor 488 goat anti-mouse IgG (H+L), highly cross-adsorbed | Mouse BrdU Ab | 1:100 |
| 5 | Alexa fluor 350 goat anti-rabbit IgG (H+L), highly cross-adsorbed | Rabbit ssDNA Ab | 1:100 |
| 6 | Alexa fluor 594 goat anti-rat IgG (H+L) | Rat BrdU Ab | 1:100 |
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Store all antibodies at 4°C. Prepare all antibody dilutions in blocking agent.
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CldU primary antibody (rat) cross-reacts with the secondary antibody against mouse and rabbit; therefore, always use highly cross-adsorbed secondary antibodies against mouse and rabbit.
