Infecting Mouse Embryos by Coculturing with Lentivirus
Abstract
Lentiviral vectors make it possible to produce transgenic animals without the need to purchase expensive microinjection equipment because integration of the transgene is possible by coculture of embryos with virus. This protocol describes the infection of fertilized oocytes with lentivirus by coculture.
Footnotes
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From the Manipulating the Mouse Embryo collection, edited by Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, and Andras Nagy.
