Protocol

Cranial Neural Crest Explants

  1. Dominique Alfandari
  1. Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003
  1. ↵1Correspondence: hcousin{at}vasci.umass.edu

Abstract

The cranial neural crest (CNC) explant assay was originally designed to assess the basic requirements for CNC migration in vitro. This protocol describes the key parameters of CNC explants in Xenopus laevis, with a focus on how to extirpate CNC cells and assay their migration in vitro. The protocol can be adapted according to the needs of the experimenter, some examples of which are discussed here.

Footnotes

  • From the Xenopus collection, edited by Hazel L. Sive.

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  1. Published in Advance January 10, 2018, doi: 10.1101/pdb.prot097394 Cold Spring Harb Protoc 2018: pdb.prot097394- © 2018 Cold Spring Harbor Laboratory Press
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