Protocol

Screening for Good Batches of Fetal Bovine Serum for Myeloma and Hybridoma Growth

Abstract

Not all lots of fetal bovine serum (FBS) are good at supporting hybridoma growth at low density. The key constituents that distinguish good batches of serum from bad are not known. It is necessary to order test batches from several suppliers and screen them as described here or purchase prescreened serum directly from the distributor.

Footnotes

  • From the Antibodies collection, edited by Edward A. Greenfield.

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