Recipe

Lysis Buffer

Reagent Final concentration
NaH2PO4 (pH 8.0)   50 mm
NaCl   300 mm
Imidazole   10 mm
Triton X-100   0.05%
EDTA   1 mm
DTT   1 mm
Lysozyme 1 mg/mL
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  1. doi:10.1101/pdb.rec098723 Cold Spring Harb Protoc 2018: pdb.rec098723- © 2018 Cold Spring Harbor Laboratory Press
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