Recipe

Washing Buffer Solution

Reagent Final concentration
NaH2PO4/Na2HPO4 (pH 8.0)  50 mm
NaCl 300 mm
Imidazole  20 mm
Triton ×-100  0.05%
EDTA  1 mm
DTT  1 mm
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  1. doi:10.1101/pdb.rec098764 Cold Spring Harb Protoc 2018: pdb.rec098764- © 2018 Cold Spring Harbor Laboratory Press
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