Long PCR Buffer (10×)
| Reagent | Final concentration |
|---|---|
| Tris-Cl (pH 9.0 at room temperature) | 500 mm |
| Ammonium sulfate | 160 mm |
| MgCl2 | 25 mm |
| Bovine serum albumin | 1.5 mg/mL |
| Use this with mixtures of DNA polymerases. Buffers supplied by the manufacturer and suitable for use with thermostable DNA polymerases may be used in place of the above buffer. | |
