Ligation Adjustment Buffer (5×)
| Reagent | Quantity (for 1 L) | Final concentration |
|---|---|---|
| Potassium acetate (1 m, pH 7.0) | 40 mL | 40 mm |
| CaCl2 (2 m) | 200 mL | 400 mm |
| MnCl2 (1 m) | 100 mL | 100 mm |
| Glycerol | 468 mL | 46.8% (v/v) |
| Add H2O to 988 mL. Adjust the pH by adding 12.8 mL of a 10% solution of acetic acid. | ||
