Production of High-Titer Retrovirus and Lentivirus Vectors
Abstract
The most commonly used method for production of retrovirus and lentivirus vectors in research laboratories is of transient transfection of 293T cells with a lentivirus vector plasmid, packaging genome plasmid(s), and an envelope expression plasmid. High-titer stocks are obtained by concentration of vector supernatants by ultracentrifugation.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
