Recipe

Lyticase Solution

10 mm Tris (pH7.2)

50 mm EDTA

1 mg/mL lyticase (Sigma-Aldrich) or 1 mg/mL Zymolyase 20T (MP Biomedicals)

Make fresh for each application.

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  1. doi:10.1101/pdb.rec094284 Cold Spring Harb Protoc 2018: pdb.rec094284- © 2018 Cold Spring Harbor Laboratory Press

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