HBS Solution (2×)
| Reagent | Quantity (1 L) | Final concentration |
|---|---|---|
| NaCl | 16.4 g | 280 mm |
| HEPES (C8H18N2O4S) | 11.9 g | 50 mm |
| Na2HPO4·7H2O | 0.38 g | 1.42 mm |
| H2O | to 1 L | |
| Adjust pH to 7.05 with 10 m NaOH. Sterilize by passing through a 0.22-μm filter and store at room temperature. | ||
