Modified Barth's Solution (MBS)
| Reagent | Final concentration (1×) |
|---|---|
| NaCl | 88 mm |
| KCl | 1 mm |
| NaHCO3 | 2.4 mm |
| MgSO4·7H2O | 0.82 mm |
| Ca(NO3)2·4H2O | 0.33 mm |
| CaCl2·2H2O | 0.41 mm |
| HEPES | 5 mm |
| Prepare a stock solution (5×, pH 7.4), which can be stored for several months at −20°C. Prepare working solutions by dilution, and autoclave prior to use. After autoclaving, add gentamycin (final concentration in 1× solution, 50 µg/mL). | |
