Bath Solution (Ca2+-, Mg2+-Free)
| Reagent | Quantity | Final concentration |
|---|---|---|
| KOH | 7.854 g | 140 mm |
| HEPES | 4.766 g | 20 mm |
| KCl | 0.1491 g | 2 mm |
| EGTA | 1.902 g | 5 mm |
| Methanesulfonic acid | 13.314 g | 140 mm |
| H2O | to 1 L | |
| Use methanesulfonic acid to adjust the pH to 7.1–7.2. Store for up to 3 years at room temperature (22°C–24°C). | ||
