Recipe

ND96 Solution

Reagent Quantity Final concentration
NaCl   5.61 g   96 mm
KCl  0.1491 g    2 mm
CaCl2·2H2O  0.2646 g   1.8 mm
MgCl2·6H2O  0.2033 g   1 mm
HEPES   4.766 g   20 mm
Na pyruvate   0.276 g  2.5 mm
Penicillin–streptomycin 100,000 U 100 U/mL
H2O    to 1 L
Use 4 m NaOH to adjust the pH to 7.5–7.6. Store for 6 mo at 4°C.
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  1. doi:10.1101/pdb.rec103770 Cold Spring Harb Protoc 2018: pdb.rec103770- © 2018 Cold Spring Harbor Laboratory Press
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