ND96 Solution
| Reagent | Quantity | Final concentration |
|---|---|---|
| NaCl | 5.61 g | 96 mm |
| KCl | 0.1491 g | 2 mm |
| CaCl2·2H2O | 0.2646 g | 1.8 mm |
| MgCl2·6H2O | 0.2033 g | 1 mm |
| HEPES | 4.766 g | 20 mm |
| Na pyruvate | 0.276 g | 2.5 mm |
| Penicillin–streptomycin | 100,000 U | 100 U/mL |
| H2O | to 1 L | |
| Use 4 m NaOH to adjust the pH to 7.5–7.6. Store for 6 mo at 4°C. | ||
