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The Basic Polymerase Chain Reaction (PCR)

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot095117

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Hot Start Polymerase Chain Reaction (PCR)

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot095125

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Touchdown Polymerase Chain Reaction (PCR)

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot095133

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ChIP–Quantitative Polymerase Chain Reaction (ChIP-qPCR)

Tae Hoon Kim and
Job Dekker

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot082628

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ChIP-chip

Tae Hoon Kim and
Job Dekker

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot082636

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ChIP-seq

Tae Hoon Kim and
Job Dekker

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot082644

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Isolation of DNA from Mouse Tails without Extraction by Organic Solvents

Michael R. Green and
Joseph Sambrook

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot093526

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Mouse Embryo Cryopreservation by Slow Freezing

Robert Taft

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot094540

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Mouse Embryo Cryopreservation by Rapid Cooling

Jillian Shaw

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot094557

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Mouse Embryo Cryopreservation by High-Osmolality Vitrification

Keiji Mochida

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot094565

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Fluorescence In Situ Hybridization of Cryosectioned Xenopus Oocytes

Christopher R. Neil and
Kimberly Mowry

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot097030

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Making Weak Antigens Strong: Modifying Antigens by Dinitrophenol or Arsynyl Coupling

Edward A. Greenfield,
James DeCaprio,
and Mohan Brahmandam

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot099952

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Making Weak Antigens Strong: Modifying Protein Antigens by Denaturation

Edward A. Greenfield,
James DeCaprio,
and Mohan Brahmandam

Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.prot099960

TOPIC INTRODUCTIONS

Introduction to Fission Yeast as a Model System

Chromatin Immunoprecipitation (ChIP) Analysis of Protein–DNA Interactions

PROTOCOLS

The Basic Polymerase Chain Reaction (PCR)

Hot Start Polymerase Chain Reaction (PCR)

Touchdown Polymerase Chain Reaction (PCR)

ChIP–Quantitative Polymerase Chain Reaction (ChIP-qPCR)

ChIP-chip

ChIP-seq

Isolation of DNA from Mouse Tails without Extraction by Organic Solvents

Mouse Embryo Cryopreservation by Slow Freezing

Mouse Embryo Cryopreservation by Rapid Cooling

Mouse Embryo Cryopreservation by High-Osmolality Vitrification

Fluorescence In Situ Hybridization of Cryosectioned Xenopus Oocytes

Making Weak Antigens Strong: Modifying Antigens by Dinitrophenol or Arsynyl Coupling

Making Weak Antigens Strong: Modifying Protein Antigens by Denaturation

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