Corrigendum: Detection of Apoptotic Cells Using Propidium Iodide Staining
Cold Spring Harb Protoc 2014; doi: 10.1101/pdb.prot082545
When this article was first published, the quantity of NH4Cl in the ACK Lysis Buffer recipe was incorrectly listed as 8.26 g. The authors apologize for this error. The correct quantity of NH4Cl to be used in the ACK Lysis Buffer is 8.02 g, which will produce a final concentration of 150 mm NH4Cl. A corrected version of the recipe is below. In addition, the HTML version of the recipe (doi: 10.1101/pdb.rec083295) and the PDF version of the article have been amended accordingly.
ACK Lysis Buffer
| Reagent | Quantity (for 1000 mL) | Final concentration |
|---|---|---|
| NH4Cl | 8.02 g | 150 mm |
| KHCO3 | 1 g | 10 mm |
| Na2EDTA | 37.2 mg | 0.1 mm |
| Dissolve all reagents in 850 mL of H2O. Adjust the pH to 7.2–7.4, and add H2O to 1000 mL. Store for up to 6 mo at room temperature. | ||
