Recipe

Extract Buffer (XB) Salts (20×)

Reagent Quantity Concentration
KCl 149.12 g  2 m
CaCl2  0.294 g  2 mm
MgCl2 (2 m; e.g., Sigma-Aldrich 68475)  10 mL 20 mm
Combine with MilliQ H2O to a volume of 1 L. Autoclave and store at 4°C.
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  1. doi:10.1101/pdb.rec103499 Cold Spring Harb Protoc 2018: pdb.rec103499- © 2018 Cold Spring Harbor Laboratory Press
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