Recipe

CSF-XB

Reagent Quantity
XB (extract buffer) 400 mL
EGTA stock (K salt; 0.5 m; pH 7.7; filter-sterilized and stored in aliquots at –20°C)  4 mL
MgCl2 solution (2 m; e.g., Sigma-Aldrich 68475) 200 µL
Make fresh on the day of extract preparation.
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  1. doi:10.1101/pdb.rec103556 Cold Spring Harb Protoc 2018: pdb.rec103556- © 2018 Cold Spring Harbor Laboratory Press
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