Recipe

CSF-XB Solution #2 for X. tropicalis Eggs

Reagent Volume
Extract Buffer (XB) salts (20×) 50 mL
Sucrose (2 m; filter-sterilized and stored in aliquots at −20°C) 25 mL
K-HEPES (1 m; K salt; pH 7.7; filter-sterilized and stored in aliquots at −20°C) 10 mL
MgCl2 (2 m; e.g., Sigma-Aldrich 68475)  1 mL
EGTA (0.5 m; K salt; pH 7.7; filter-sterilized and stored in aliquots at –20°C) 20 mL
Make fresh on the day of extract preparation. Combine and bring to 1 L with MilliQ H2O. Adjust pH to 7.7 with KOH.
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  1. doi:10.1101/pdb.rec103564 Cold Spring Harb Protoc 2018: pdb.rec103564- © 2018 Cold Spring Harbor Laboratory Press
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