Recipe

XB Buffer (10×)

1 m KCl

1 mm CaCl2

10 mm MgCl2

500 mm sucrose

100 mm HEPES-KOH pH 7.8

Store at 4°C and dilute to 1× with ddH2O before use.

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  1. doi:10.1101/pdb.rec103580 Cold Spring Harb Protoc 2018: pdb.rec103580- © 2018 Cold Spring Harbor Laboratory Press
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