Recipe

Extract Fix

Glycerol 60% (v/v)
Marc's modified Ringer's (MMR; 20×)    1×
Hoechst (for live imaging of DNA, use Sigma-Aldrich BisBenzimide H 33342 trihydrochloride, B2261)  1 µg/mL
Formaldehyde    10%
Prepare fresh.
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  1. doi:10.1101/pdb.rec103663 Cold Spring Harb Protoc 2018: pdb.rec103663- © 2018 Cold Spring Harbor Laboratory Press

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