Recipe

Frog Husbandry Water

Instant Ocean Sea Salt (Instant Ocean)  0.88 g/L
Cichlid Lake Salt (SeaChem)  0.33 g/L
Alkaline Buffer (SeaChem)  0.84 g/L
Acidic Buffer (SeaChem)  0.33 g/L
Equilibrium Buffer (SeaChem) 0.033 g/L
Prepare fresh. Dissolve components in double-distilled, reverse-osmosis, or carbon-filtered water. Sterilization is not required. Adjust the amounts of salts and buffers to ensure frog husbandry water quality parameters fall within acceptable ranges. For example, in some X. laevis aquatic husbandry systems, water temperature is kept at ∼18°C, pH at 7.0–7.5, conductivity at 1.2–1.8 mS, and hardness at ∼200 ppm (CaCO3).
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  1. doi:10.1101/pdb.rec103853 Cold Spring Harb Protoc 2018: pdb.rec103853- © 2018 Cold Spring Harbor Laboratory Press

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