Recipe

YAC Equilibration Buffer

TAE buffer   1×
NaCl 100 mm
Spermine (Sigma-Aldrich, tetrachloride, S1141)   30 µm
Spermidine (Sigma-Aldrich, trihydrochloride, S2501)   70 µm
To obtain the 1× working solution of spermine and spermidine, use polyamine mix (1000×).
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  1. doi:10.1101/pdb.rec095257 Cold Spring Harb Protoc 2018: pdb.rec095257- © 2018 Cold Spring Harbor Laboratory Press
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