ATP Regeneration/Energy Mix
| Reagents | Quantity |
|---|---|
| Creatine phosphate (Sigma-Aldrich P7936) | 383 mg |
| ATP (Sigma-Aldrich A6419) | 110 mg |
| EGTA (potassium salt; 0.5 m; pH 7.7 with KOH; filter-sterilized and stored at 4°C) | 40 µL |
| MgCl2 (2 m) | 100 µL |
| Combine with MilliQ H2O to a volume of 10 mL. Split into 1-mL aliquots, and store at −20°C. | |
