Circularization Mix
| Reagent | Amount (for 1 sample) |
|---|---|
| Nuclease-free H2O (Ambion AM9937) | 7776 µL |
| T4 DNA ligase buffer (10×; NEB B0202S) | 1000 µL |
| Prepare the solution fresh before use. Use new ligase buffer if reagent is too old (it includes dithiothreitol, which oxidizes over time). | |
