TNE Buffer for ChIA-PET
| Reagent | Amount to add (for 500 mL) | Final concentration (1×) |
|---|---|---|
| Tris-HCl (1 m, pH 8; ThermoFisher 15568-025) | 5 mL | 10 mm |
| EDTA (0.5 m, pH 8; Ambion AM9260G) | 0.1 mL | 0.1 mm |
| NaCl (5 m; Sigma-Aldrich S3014) | 5 mL | 50 mm |
| Nuclease-free H2O (Ambion AM9937) | 489.9 mL | |
| Store at room temperature for up to 1 mo. | ||
