Recipe

Polymerase I Master Mix

Reagent Amount (for 1.5 samples)
Nuclease-free H2O (Ambion AM9937) 57.8 µL
NEB buffer 2 (10×; New England Biolabs B7002S)  7.5 µL
dNTP Mix (10 mm; Eppendorf 0032 003.109) 3.75 µL
Prepare the solution fresh before use.
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  1. doi:10.1101/pdb.rec104919 Cold Spring Harb Protoc 2018: pdb.rec104919- © 2018 Cold Spring Harbor Laboratory Press
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