Binding and Washing (B&W) Buffer (2×)
| Reagent | Amount (for 100 mL) | Final concentration (2×) |
|---|---|---|
| Tris-HCl (1 m, pH 7.5; ThermoFisher 15567-027) | 1 mL | 10 mm |
| EDTA (0.5 m, pH 8; Ambion AM9260G) | 200 µL | 1 mm |
| NaCl (5 m; Sigma-Aldrich S3014) | 40 mL | 2 m |
| Nuclease-free H2O (Ambion AM9937) | 58.8 mL | |
| Store the buffer at room temperature. | ||
