PCR Master Mix for ChIA-PET
| Reagent | Amount (for 1 sample) |
|---|---|
| Nuclease-free H2O (Ambion AM9937) | 21 µL |
| Library construction primer 1 (10 µm; see below) | 1 µL |
| Library construction primer 2 (10 µm; see below) | 1 µL |
| Phusion High-Fidelity PCR Master Mix with HF buffer (Finnzymes F-531) | 25 µL |
| Use the following primer sequences: | |
| Library construction-primer 1: 5′-AATGATACGGCGACCACCGAGATCTACACCCTATCCCCTGTGTGCCTTG-3′ | |
| Library construction-primer 2:5′-CAAGCAGAAGACGGCATACGAGATCGGTCCATCTCATCCCTGCGTGTC-3′ | |
| Prepare the solution fresh before use. | |
