Cover image

Immunofluorescence image of beads coated with reconstituted chromatin containing the centromere-specific histone CENP-A (red), which assembles functional centromeres and kinetochores (green) upon incubation in Xenopus egg extract. This issue includes a protocol describing techniques for the biochemical dissection of centromere function using this system (doi: 10.1101/pdb.prot102509). Image courtesy of Julio C. Flores Servin, Bradley T. French, and Aaron F. Straight.