Surgical Artificial Insemination in Mice

Abstract

Artificial insemination can be achieved by directly adding sperm from a particular male into the oviduct of a female successfully bred with a vasectomized male by a surgical procedure. Those who are comfortable performing oviduct embryo transfers might find this approach much easier than delivering the sperm into the vagina. Multiple females can be inseminated with sperm from a single male to rescue the line, expand the line quickly, or generate relatively synchronous embryos.