Recipe

5C Annealing Buffer (10×)

Reagent Stock solution Quantity (for 10 mL) Final concentration
Tris-acetate (pH 7.9) 1 m  2 mL 200 mm
Potassium acetate 1 m  5 mL 500 mm
Magnesium acetate 1 m  1 mL 100 mm
DTT 1 m 100 µL  10 mm
H2O  1.9 mL
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  1. doi:10.1101/pdb.rec099200 Cold Spring Harb Protoc 2018: pdb.rec099200- © 2018 Cold Spring Harbor Laboratory Press
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