Histone H1 Kinase Assay Cocktail
| Reagent | Amount to add |
|---|---|
| Histone H1 | 5 mg |
| β-glycerophosphate | 111 mg |
| 1 m MgCl2 | 76 µL |
| 0.5 m EGTA | 204 µL |
| 50 mm ATP | 6.4 µL |
| Bring volume to 6.4 mL with H2O. Keep frozen in 500-µL aliquots. Combine with 50 µCi [γ-32P]ATP just prior to use. | |
