Protocol

Preparing and Using Adjuvants

Abstract

Nonspecific stimulators of the immune response are known as adjuvants. The judicious use of adjuvants is essential to induce a strong antibody response to soluble antigens. The most commonly used adjuvant for research work is Freund's adjuvant. Described here is the preparation and use of Freund's adjuvant as well as four alternative adjuvants: Ribi, Hunter's TiterMax, Magic Mouse, and aluminum hydroxide.

Footnotes

  • From the Antibodies collection, edited by Edward A. Greenfield.

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