Steinberg's Solution (10×, pH 7.4)
| Reagent | Amount |
|---|---|
| NaCl | 34 g |
| KCl | 0.5 g |
| Ca(NO3)2·4H2O | 0.8 g |
| MgSO4·7H2O | 2.06 g |
| Tris | 5.6 g |
| HCl | 3.3 mL |
| Phenol red | 3 mg |
| H2O | to 1 L |
| Sterilize by autoclaving (for 20 min at 121°C), and store for several months to years at room temperature. | |
