Binding Buffer for Oligo(dT) Beads
| Reagent | Quantity (for 100 mL) | Final concentration |
|---|---|---|
| LiCl (5 m) | 10 mL | 0.5 m |
| Tris-Cl (1 m, pH 8.0) | 10 mL | 0.1 m |
| EDTA (0.5 m) | 2 mL | 0.01 m |
| Lithium dodecylsulfate (10%, w/v) | 10 mL | 1% (w/v) |
| Dithiothreitol (1 m) | 500 µL | 5 mm |
| If any precipitation is observed, warm to room temperature and shake until all the components are fully resuspended. | ||
