Large-Scale Preparation and Linearization of Bacterial Artificial Chromosome (BAC) DNA
Abstract
A critical step for creating bacterial artificial chromosome (BAC) transgenic organisms is the production of high-quality BAC DNA. The method described here provides a source of DNA for further manipulation and modification, but can also be used for preparing modified BAC DNA, resulting from the one-step or two-step strategy, for use in other applications such as BAC transgenesis. An analysis of precautions to take when working with large DNA molecules is also provided.
Footnotes
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From the Molecular Cloning collection, edited by Michael R. Green and Joseph Sambrook.
